Estrogen Kit ELISA

N° du produit ABIN5524301

Ce kit ELISA Rat Estrogen est un kit ELISA Colorimetric conçu pour quantifier Rat Estrogen.

Aperçu rapide pour Estrogen Kit ELISA (ABIN5524301)

Antigène: Estrogen

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Détail du produit Estrogen Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: For quantitative detection in serum, plasma, tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Sensibilité: 9.375 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard
• Standard Dilution Buffer
• Biotin-labeled Antibody
• Purified water
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Competitive ELISA, Coated with Antigen

Volume d'échantillon: 50 μL

Plaque: Pre-coated

Préparation des réactifs:

• Bring all reagents and samples to room temperature for 20 minutes before use.
• Wash Buffer: If crystals have formed in the concentrate, you can warm it with 40 °C water bath Concentrated Wash Buffer into 750 mL Wash Buffer with deionized or distilled water. Put unused solution back at 2-8 °C.
• Standards:
  1. Add 1 mL Sample Dilution Buffer into one Standard tube (labeled as zero tube), keep the tube at room temperature for 10 minutes and mix them thoroughly. Note: If the standard tube concentration higher than the range of the kit,please dilute it and labeled as zero tube.
  2. Label 7 EP tubes with 1/2, 1/4, 1/8, 1/16, 1/32, 1/64 and blank respectively. Add 0.3 mLof the Sample Dilution Buffer into each tube. Add 0.3 mLof the above Standard solution (from zero tube) into 1st tube and mix them thoroughly. Transfer 0.3 mL from 1st tube to 2nd tube and mix them thoroughly. Transfer 0.3 mL from 2nd tube to 3rd tube and mix them thoroughly, and so on. Sample Dilution Buffer was used for the blank control. Note: It is best to use Standard Solutions within 2 hours.
• Preparation of Biotin-labeled Antibody Working Solution:
  Prepare it within 1 hour before experiment.
  1. Dissolve: Add 70ul purified water into tube and mix them thoroughly, after the biotin-labeled antibody is dissolved, please store it at 2-8°C.
  2. Calculate required total volume of the working solution: 0.05ml/well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  3. Dilute the Biotin-detection antibody with Antibody Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL Biotin-labeled antibody into 99 µL Antibody Dilution Buffer.)
• Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution:
  Prepare it within 30 minutes before experiment.
  1. Calculate required total volume of the working solution: 0.1ml/well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  2. Dilute the SABC with SABC Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL of SABC into 99 µL of SABC Dilution Buffer.)

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4 °C for 6 months

Date de péremption: 6 months

Détail du antigène

Antigène: Estrogen

Classe de substances: Hormone